Association of Endothelin-1 rs5370 G>T gene polymorphism with the risk of nephrotic syndrome in children
| Autor: | Mohammad Hashemi, Simin Sadeghi-Bojd, Soheib Aryanezhad, Maryam Rezaei |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Journal of Nephropathology, Vol 6, Iss 3, Pp 138-143 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2251-8363 2251-8819 |
| DOI: | 10.15171/jnp.2017.24 |
| Popis: | Background: Primary nephrotic syndrome (NS) is a common kidney disease in children. Objectives: The present study was aimed to investigate whether rs5370 G>T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS. Patients and Methods: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G>T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method. Results: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G>T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G>T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels. Conclusions: In conclusion, our findings indicate that ET-1 rs5370 G>T is not associated with NS. Further studies with larger sample sizes and different ethnicities are required to validate our findings. |
| Databáze: | Directory of Open Access Journals |
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