Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis

Autor: Ying Ying, Chao Ma, Yajie Zhang, Xiaoping Li, Hongxin Wu
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Polish Journal of Food and Nutrition Sciences, Vol 73, Iss 1, Pp 70-79 (2023)
Druh dokumentu: article
ISSN: 2083-6007
DOI: 10.31883/pjfns/160179
Popis: In this study, a novel neutral non-starch polysaccharide (GP1A) was extracted using hot water from ginseng roots. Chemical characteristics, monosaccharide compositions and structure of GP1A were investigated using scanning electron microscopy, gel permeation chromatography, X-ray diffraction, Fourier transform infrared spectroscopy, gas chromatography with mass spectrometer, one-dimensional and two-dimensional nuclear magnetic resonance. The results indicated that the lyophilized GP1A was a homogeneous polysaccharide with a low molecular weight of 1.03 kDa. The dominating monosaccharides of GP1A were D-glucose, D-galactose, D-mannose, D-xylose and D-arabinose. The structure analysis indicated the main chain residues sequence of GP1A was α-D-Glc p -(6→3)-α-D-Gal p -(1→1)-α-D-Glc p -(3→4)-β-D-Man p -(2→2)-β-D-Ara p with branch chains of α-D-Glc p substituted at α-D-Gal p and β-D-Xyl p substituted at β-D-Manp.
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