| Popis: |
Dalia Mahmoud Eldewi,1 Alshaymaa M Alhabibi,1 Hanaa Mohammed Eid El Sayed,2 Sammar Ahmed Kasim Mahmoud,2 Sanaa Mohammed El Sadek,3 Rasha Mahmoud Gouda,3 Mohammed Abd El Malik Hassan,4 Amal H Ibrahim,2 Naglaa F Abd El Haliem5 1Clinical Pathology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 2Internal Medicine Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 3Pediatric Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 4Pediatric Department, Faculty of Medicine for Boys, Al-Azhar University, Cairo, Egypt; 5Medical Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, EgyptCorrespondence: Alshaymaa M AlhabibiClinical Pathology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo 11765, EgyptTel +20 100 289 4075Email Elshymaamohamed.medg@azhar.edu.egBackground: Altered regulation of the complement system is associated with multiple kidney diseases. CD35, CD55 and CD59 regulate the complement system, and changes in their expression have previously been linked with kidney disease. This study assessed whether changes in the expression levels of these proteins are associated specifically with chronic kidney disease (CKD) to understand its pathogenesis.Materials and methods: Sixty CKD patients and 60 age-matched controls were enrolled and divided into two groups: Group I (n=30 pediatric patients and n=30 controls) and Group II (n=30 adult patients and n=30 controls). The expression of CD35, CD55 and CD59 on peripheral blood cells was evaluated by flow cytometry as the proportion of positive cells expressing the marker and mean fluorescence intensity (MFI), also the relation of these markers to the stage of CKD was also evaluated.Results: Pediatric and adult CKD patients had significantly lower proportion of erythrocytes expressing CD35, CD55 and CD59 than healthy controls (P |
