| Autor: |
Ilaria Benedetti, Pablo I. Nikel, Víctor de Lorenzo |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Data in Brief, Vol 6, Iss , Pp 738-744 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2352-3409 |
| DOI: |
10.1016/j.dib.2016.01.022 |
| Popis: |
Engineering of robust microbial cell factories requires the use of dedicated genetic tools somewhat different from those traditionally used for laboratory-adapted microorganisms. We have edited and formatted the ChnR/PchnB regulatory node of Acinetobacter johnsonii to ease the targeted engineering of ectopic gene expression in Gram-negative bacteria. The proposed compositional standard was thoroughly verified with a monomeric and superfolder green fluorescent protein (msf•GFP) in Escherichia coli. The expression data presented reflect a tightly controlled transcription initiation signal in response to cyclohexanone. Data in this article are related to the research paper “Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes” [1]. Keywords: Expression system, Heterologous gene expression, Metabolic engineering, Synthetic biology, Escherichia coli, Pseudomonas putida |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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