Centriolar satellites associate with condensed chromatin in early mouse oocytes and undergo redistribution during transition to dictyate

Autor: Valentina Prodanova Hadzhinesheva, Irina Valcheva Chakarova, Stefka Metodieva Delimitreva, Maya Dyankova Markova, Venera Pantaleeva Nikolova, Milena Sergeeva Mourdjeva, Pavel Istiliyanov Rashev, Ralitsa Stefanova Zhivkova
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Biotechnology & Biotechnological Equipment, Vol 32, Iss 6, Pp 1622-1626 (2018)
Druh dokumentu: article
ISSN: 1310-2818
1314-3530
13102818
DOI: 10.1080/13102818.2018.1541761
Popis: In eukaryotic cells during interphase, the centrosome is positioned close to the nucleus by a yet unidentified mechanism and determines the basic cell polarity. The aim of this study was to trace the dynamics of centriolar satellite protein PCM-1 in newborn mouse ovaria. Ovarian sections of newborn mice were subjected to immunofluorescent analysis. The intracellular localization of PCM-1 in prophase I oocytes was compared between 1- and 2-day-old mice. In oocytes from 1-day-old ovaria, double or broader single discrete PCM-1 aggregates with perinuclear localization were observed in association with condensed chromatin regions. In oocytes from 2-day-old ovaria, PCM-1 structures were less discrete and chromatin was homogenous. In some oocytes, single or double PCM-1 aggregate was still recognizable, while in others, the PCM-1-containing structure was crescent-shaped. Our study describes for the first time reorganization of centriolar satellite protein PCM-1 in prophase I oocytes from developing primordial follicles correlated with chromatin changes during transition to dictyate.
Databáze: Directory of Open Access Journals
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