Sperm parameters, protamine deficiency, and apoptosis in total globozoospermia
| Autor: | Jalal Ghasemzadeh, Ali Reza Talebi, Mohammad Ali Khalili, Farzaneh Fesahat, Iman Halvaei, Ali Nabi, Sareh Ashourzadeh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Iranian Journal of Reproductive Medicine, Vol 13, Iss 8, Pp 495-502 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1680-6433 2008-2177 |
| Popis: | Background: Globozoospermia is a severe form of teratozoospermia (incidence < 0.1%) in infertile men that is characterized by round headed sperm and acrosomeless in semen. Objective: To compare the semen parameters, protamine deficiency, and apoptosis in ejaculated spermatozoa between globozoospermic and normozoospermic men. Materials and Methods: Thirty six semen samples were divided into two groups including 15 infertile men with total globozoospermic (> 90% round-headed sperm) and 21 healthy donors with normal spermograms as controls. Semen analysis was performed according to World Health Organization criteria (2010). Sperm protamine deficiency was assessed using Chromomycin A3 (CMA3) staining and the rate of apoptotic spermatozoa was evaluated with TUNEL assay. Results: Sperm concentration, motility, and normal morphology in globozoospermic men were significantly decreased compared with controls (p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|