| Autor: |
Sarah Kabui, Josephine Kimani, Caroline Ngugi, John Kagira |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Veterinary Medicine and Science, Vol 10, Iss 3, Pp n/a-n/a (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2053-1095 |
| DOI: |
10.1002/vms3.1420 |
| Popis: |
Abstract Background Ruminant mastitis continues to be a cause of economic losses in the dairy industry and remains a major public health hazard globally. Objectives This cross‐sectional study was carried out in Mukurweini Sub‐County of Nyeri County, Kenya, to investigate the prevalence of bacteria causing mastitis, risk factors associated with goat mastitis and the antibiotic resistance profiles of bacteria isolated from the goat milk. Methods Farm level data on risk factors for mastitis was obtained from 56 farmers using a semi structured questionnaire. A total of 189 goat milk samples were collected. The goat's udder was observed for signs of clinical mastitis and the California Mastitis Test (CMT) used to test the milk for sub‐clinical mastitis. All samples were then cultured for morphological identification of bacteria and strain typing by Matrix Assisted Laser Desorption/Ionization (MALDI)‐Time of Flight (ToF) technique. Antimicrobial susceptibility of the isolated Staphylococcus aureus, coagulase‐negative Staphylococcus (CoNS), Escherichia coli, Klebsiella oxytoca, Pseudomonas spp., Enterobacter spp., Proteus vulgaris and Escherichia vulneris to eight commonly used antibiotics was done by the disc diffusion method and validated by determining the presence of antibiotic resistance genes (mecA and blaTEM) using polymerase chain reaction method. Results The prevalence of clinical mastitis was 1.1% (2/189) while that of sub‐clinical mastitis was 84.7% (160/189). Higher (p |
| Databáze: |
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