Identification and characterization of a heterotrimeric archaeal DNA polymerase holoenzyme

Autor: Jiangyu Yan, Thomas R. Beattie, Adriana L. Rojas, Kelly Schermerhorn, Tamzin Gristwood, Jonathan C. Trinidad, Sonja V. Albers, Pietro Roversi, Andrew F. Gardner, Nicola G. A. Abrescia, Stephen D. Bell
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/ncomms15075
Popis: The current model for B-family DNA polymerases in archaea is one of single-subunit enzymes in contrast to the multi-subunit complexes in eukaryotes. Here the authors show that PolB1 fromSulfolobus solfataricusexists as a heterotrimeric complex in cell extracts.
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