Investigation of the Low-Populated Excited States of the HIV-1 Nucleocapsid Domain

Autor: Assia Mouhand, Loussiné Zargarian, Anissa Belfetmi, Marjorie Catala, Marco Pasi, Ewen Lescop, Carine Tisné, Olivier Mauffret
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Viruses, Vol 14, Iss 3, p 632 (2022)
Druh dokumentu: article
ISSN: 14030632
1999-4915
DOI: 10.3390/v14030632
Popis: The nucleocapsid domain (NCd), located at the C-terminus of the HIV-1 Gag protein, is involved in numerous stages of the replication cycle, such as the packaging of the viral genome and reverse transcription. It exists under different forms through the viral life cycle, depending on the processing of Gag by the HIV-1 protease. NCd is constituted of two adjacent zinc knuckles (ZK1 and ZK2), separated by a flexible linker and flanked by disordered regions. Here, conformational equilibria between a major and two minor states were highlighted exclusively in ZK2, by using CPMG and CEST NMR experiments. These minor states appear to be temperature dependent, and their populations are highest at physiological temperature. These minor states are present both in NCp7, the mature form of NCd, and in NCp9 and NCp15, the precursor forms of NCd, with increased populations. The role of these minor states in the targeting of NCd by drugs and its binding properties is discussed.
Databáze: Directory of Open Access Journals
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