Molecular epidemiology and whole genome sequencing analysis of clinical Mycobacterium bovis from Ghana.

Autor: Isaac Darko Otchere, Andries J van Tonder, Adwoa Asante-Poku, Leonor Sánchez-Busó, Mireia Coscollá, Stephen Osei-Wusu, Prince Asare, Samuel Yaw Aboagye, Samuel Acquah Ekuban, Abdallah Iddrisu Yahayah, Audrey Forson, Akosua Baddoo, Clement Laryea, Julian Parkhill, Simon R Harris, Sebastien Gagneux, Dorothy Yeboah-Manu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: PLoS ONE, Vol 14, Iss 3, p e0209395 (2019)
Druh dokumentu: article
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0209395
Popis: BACKGROUND:Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a re-emerging problem in both livestock and humans. The association of some M. bovis strains with hyper-virulence, MDR-TB and disseminated disease makes it imperative to understand the biology of the pathogen. METHODS:Mycobacterium bovis (15) among 1755 M. tuberculosis complex (MTBC) isolated between 2012 and 2014 were characterized and analyzed for associated patient demography and other risk factors. Five of the M. bovis isolates were whole-genome sequenced and comparatively analyzed against a global collection of published M. bovis genomes. RESULTS:Mycobacterium bovis was isolated from 3/560(0.5%) females and 12/1195(1.0%) males with pulmonary TB. The average age of M. bovis infected cases was 46.8 years (7-72years). TB patients from the Northern region of Ghana (1.9%;4/212) had a higher rate of infection with M. bovis (OR = 2.7,p = 0.0968) compared to those from the Greater Accra region (0.7%;11/1543). Among TB patients with available HIV status, the odds of isolating M. bovis from HIV patients (2/119) was 3.3 higher relative to non-HIV patients (4/774). Direct contact with livestock or their unpasteurized products was significantly associated with bTB (p
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