Separation of digoxin by luiquid-luiquid extraction from extracts of foxglove secondary glycosides
| Autor: | Novković Vesna V., Stanojević Ljiljana P., Cakić Milorad D., Veljković Vlada B., Stanković Mihajlo Z. |
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| Jazyk: | English<br />Serbian |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Hemijska Industrija, Vol 68, Iss 2, Pp 161-170 (2014) |
| Druh dokumentu: | article |
| ISSN: | 0367-598X 2217-7426 |
| DOI: | 10.2298/HEMIND130422040N |
| Popis: | The present study deals with the extraction of digoxin (Dgx) from chloroform and trichloroethylene extracts of the secondary glycosides of fermented foxglove (Digitalis lanata Ehrh.) foliage by liquid-liquid extraction. The extraction degree (ED) of Dgx achieved by maceration and percolation using 10% vol. aqueous ethanol solutions were higher than 95%. Using trichlorethylene and chloroform, the ED of Dgx of about 100% and 96%, respectively from the liquid ethanolic extracts (macerate or percolate) were achieved by the four-cycle extraction. Fifteen separating funnels were employed for the liquid-liquid extraction. Three different four-component two-phase systems (ethanol:water - chloroform:ethyl acetate, ethanol:water - chloroform:trichloroethylene and ethanol:water - trichloroethylene:ethyl acetate) were tested as an extracting solvent to get the final product having more than 98% of Dgx. The initial amount of the chloroform or trichloroethylene extract in the light phase was varied between 5 and 25 g/L, while the volume ratio of light and heavy phases was in the range of 1:1 to 1:2. The best Dgx yield of 98% was achieved with the system ethanol:water - chloroform:trichloroethylene 35:15:20:30 at the volume ratio of the phases of 1:1.1 and at the initial amount of the extract of 15 g/L. Purity of the separated digoxin was 99.8 %. [Projekat Ministarstva nauke Republike Srbije, br. TR-34012] |
| Databáze: | Directory of Open Access Journals |
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