Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice

Autor: Weilan Chen, Xiaoling Hu, Li Hu, Xinyue Hou, Zhengyan Xu, Fanmin Yang, Min Yuan, Feifan Chen, Yunxiao Wang, Bin Tu, Ting Li, Liangzhu Kang, Shiwen Tang, Bingtian Ma, Yuping Wang, Shigui Li, Peng Qin, Hua Yuan
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Rice, Vol 15, Iss 1, Pp 1-6 (2022)
Druh dokumentu: article
ISSN: 1939-8425
1939-8433
DOI: 10.1186/s12284-022-00601-4
Popis: Abstract Background: Grain size is a direct determinant of grain weight and yield in rice; however, the genetic and molecular mechanisms determining grain size remain largely unknown. Findings: We identified a mutant, wide grain 3 (wg3), which exhibited significantly increased grain width and 1000-grain weight. Cytological analysis showed that WG3 regulates grain size by affecting cell proliferation. MutMap-based gene cloning and a transgenic experiment demonstrated that WG3 encodes a GRAS protein. Moreover, we found that WG3 directly interacts with DWARF AND LOW-TILLERING (DLT), a previously reported GRAS protein, and a genetic experiment demonstrated that WG3 and DLT function in a common pathway to regulate grain size. Additionally, a brassinosteroid (BR) sensitivity test suggested that WG3 has a positive role in BR signaling in rice. Collectively, our results reveal a new genetic and molecular mechanism for the regulation of grain size in rice by the WG3-DLT complex, and highlight the important functions of the GRAS protein complex in plants. Conclusion: WG3 functions directly in regulating grain size and BR signaling in rice.
Databáze: Directory of Open Access Journals
Externí odkaz: