3,3′-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence

Autor:	Murtaza Shabbir-Hussain, Mitch Trauernicht, Alessandro D’Urso, Andrea E. Holmes, Milan Balaz, Jung Kyu Choi
Jazyk:	angličtina
Rok vydání:	2011
Předmět:	Cy7 cyanine dye left-handed Z-DNA circular dichroism DNA recognition sensing Biology (General) QH301-705.5 Chemistry QD1-999
Zdroj:	International Journal of Molecular Sciences, Vol 12, Iss 11, Pp 8052-8062 (2011)
Druh dokumentu:	article
ISSN:	12118052 1422-0067
DOI:	10.3390/ijms12118052
Popis:	Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3′-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)2 and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/616eee2454d14f9c9e6c6a6119de07af Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF