| Autor: |
Ruo‐Fu Tang, Wen‐Jing Li, Yun Lu, Xuan‐Xuan Wang, Su‐Yu Gao |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
ESC Heart Failure, Vol 11, Iss 2, Pp 1009-1021 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2055-5822 |
| DOI: |
10.1002/ehf2.14660 |
| Popis: |
Abstract Aims Myocardial ischaemia–reperfusion injury (MIRI) contributes to serious myocardial injury and even death. Long non‐coding RNAs (lncRNAs) have been reported to play pivotal roles in the occurrence and development of MIRI. Here, the detailed molecular mechanism of lncRNA SNHG1 in MIRI was explored. Methods and results A cell model of MIRI was established through hypoxia/reoxygenation (H/R) stimulation. Cell viability and pyroptosis were evaluated utilizing MTT, PI staining, and flow cytometry. Interleukin (IL)‐1β and IL‐18 secretion levels were examined by ELISA. The gene and protein expression were detected by RT‐qPCR and western blot, respectively. Dual luciferase reporter gene, RIP and ChIP assays were performed to analyse the molecular interactions. The results showed that lncRNA SNHG1 overexpression alleviated H/R‐induced HL‐1 cell pyroptosis (all P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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