Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown
| Autor: | Daqian Sun, Margherita Melegari, Sunandini Sridhar, Charles E. Rogler, Liang Zhu |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 41, Iss 1, Pp 59-63 (2006) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/000112203 |
| Popis: | A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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