| Autor: |
Q. Zhou, X. C. Tan, X. J. Guo, Y. J. Huang, H. Y. Zhai |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Royal Society Open Science, Vol 5, Iss 9 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2054-5703 |
| DOI: |
10.1098/rsos.180750 |
| Popis: |
We synthesized a selective molecularly imprinted solid-phase extraction (MIP-SPE) column and established an extraction and enrichment method using this MIP-SPE column. By coupling with HPLC, we developed a new method to detect trace amounts of melamine in eggs. The MIP-SPE column was synthesized by in situ thermal-initiated polymerization using melamine as the template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker and azodiisobutyronitrile as the initiator. HPLC was used to evaluate the identification and enrichment capability of the MIP-SPE column and for the measurement of melamine in the sample. The melamine concentration exhibited an excellent linear relationship in the range of 0.1–25.0 µg ml−1 (r = 0.9983). The identification capability of the MIP-SPE column was apparently superior to that of the non-imprinted polymer solid-phase extraction column; an average enrichment factor of 46.8-fold (RSD = 3.5%) was obtained for 0.4 µg ml−1 melamine by the MIP-SPE column. When the MIP-SPE HPLC method was applied to the detection of melamine in eggs, an average recovery rate of 93.5–102.0% (RSD = 3.6–4.9%) and a limit of detection of 0.05 µg kg−1 were obtained. This method is simple, fast and cost-effective; thus, it can greatly simplify the pre-treatment of complex samples and can be used in the detection of residual melamine in eggs and other products. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
