| Autor: |
David Ibáñez, Daniel Izquierdo-Bote, María Begoña González-García, David Hernández-Santos, Pablo Fanjul-Bolado |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Chemosensors, Vol 9, Iss 11, p 303 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2227-9040 |
| DOI: |
10.3390/chemosensors9110303 |
| Popis: |
A new transducer based on a screen-printed carbon electrode has been developed for the quantification of thiram. Detection of this fungicide is based on the performance of two enzymes: (1) aldehyde dehydrogenase catalyzes the aldehyde oxidation using NAD+ as a cofactor and simultaneously, (2) diaphorase reoxidizes the NADH formed in the first enzymatic process due to the presence of hexacyanoferrate(III) which is reduced to hexacyanoferrate(II). Taking into account that aldehyde dehydrogenase is inhibited by thiram, the current decreases with pesticide concentration and thiram can be electrochemically quantified below legal limits. The transducer proposed in this work involves the modification of the carbon WE with the co-factors (NAD+ and hexacyanoferrate(III)) required in the enzymatic system. The new device employed in this work allows the detection of 0.09 ppm thiram, a concentration below legal limits (Maximum Residue Limits 0.1–10 ppm). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
