| Autor: |
A. ALMÁSI, K. NEMES, R. SÁRAY, Á. GELLÉRT, N. INCZE, P. VÁGI, E. BADICS, V. SOÓS, K. SALÁNKI |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Biologia Plantarum, Vol 67, Iss 1, Pp 105-113 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1573-8264 |
| DOI: |
10.32615/bp.2023.010 |
| Popis: |
Tomato spotted wilt virus (TSWV) has significant economic impact on horticulture worldwide. One of the five proteins encoded by TSWV genome is the multifunctional NSs protein, which is a viral suppressor of RNA silencing (VSR) besides functioning as the effector of Tsw resistance gene in resistant pepper cultivars. In this study we demonstrate the in vivo self-interaction of NSs protein using bimolecular fluorescence complementation and yeast two-hybrid assays, and propose that a highly charged alpha helix located at the second half of the protein is required for self-interaction. Furthermore, we confirmed that self-interaction is not required for its effector function on pepper. Moreover, self-interaction is dispensable for gene silencing suppressor activity, although it enhances the suppression efficiency. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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