| Autor: |
Julio Cesar Ortega Cambara, Vivian León Fernández, Duniesky Martínez García, Alina Sobrino Legón, Daisy Dopico Ramírez, Enrique Rosendo Pérez Cruz |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Revista Centro Azúcar, Vol 51, Iss 2, Pp e1065(02/05/2024)-e1065(02/05/2024) (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2223-4861 |
| Popis: |
Introduction: Molasses is an agro-industrial by-product that could be used as inexpensive carbon source to reduce the culture media cost for enzyme-producing microorganisms. Objective: To evaluate four combinations of carbon and nitrogen sources for industrial production of BfrA by Pichia pastoris BfrA4X. Materials and Methods: Medium composition was evaluated in an orbital shaker and increased discontinuous cultures. The culture was scaled up to a 42-L fermenter based on a constant volumetric mass transfer coefficient (kLa). Enzyme activity and biomass yield was evaluated. Results and Discussion: When increased discontinuous cultures was carried out in a 7.5-L fermenter with MYH medium, an overall BfrA yield of 118 ± 1 U/mL of fermentation culture was obtained, similar to the yield of 120 ± 7 U/mL yield obtained for SYE medium. The culture was expanded to a 42-L fermenter, with MYH medium a higher overall BfrA activity of 167 ± 8 U/mL was obtained. Conclusions: Cane molasses is a low-cost feedstock that could be used for the economic scale-up of efficient BfrA production by P. pastoris BfrA4X, based on constant kLa. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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