| Autor: |
Wanapas Wachiradejkul, Pichayapa Sukmak, Supisara Treveeravoot, Laphatrada Yurasakpong, Nutnicha Rangchaikul, Pimngeon Chatkul, Pitsinee Supapol, Apiwan Arinno, Natnicha Teansuk, Jakkapong Inchai, Sukpapohn Phummisutthigoon, Makha Phongjit, Autsadakorn Loungjan, Nattaphong Akrimajirachoote, Wanangkan Poolsri, Chanat Aonbangkhen, Rungtiwa Khumjiang, Chatchai Muanprasat, Chutima S. Vaddhanaphuti, Pawin Pongkorpsakol |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 122, Iss , Pp 106519- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2024.106519 |
| Popis: |
Intestinal tight junction disruption initiates pathogenesis of colitis and determines diseases progression. At present, there is no therapeutics that directly corrects intestinal tight junction disassembly. Here, we discovered that Ocimum sanctum L. flower aqueous extract (OSLF) and gallic acid, increased intestinal barrier function by suppressing tight junction-dependent leak pathway permeability after being disrupted by Ca2+ depletion method. Transepithelial electrical resistance (TER) measurement, western blot analysis, immunofluorescence staining, and molecular docking indicated that OSLF and gallic acid improved intestinal barrier function by inducing tight junction assembly and inhibiting leak pathway permeability in intestinal epithelial cell monolayers via CaMKK-β/AMPK/SIRT-1/ERK-dependent fashion. Therefore, Gallic acid represents a drug candidate for treating diseases associated impaired intestinal barrier function including colitis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect