PHENOTYPIC AND GENOTYPIC ANALYSIS OF A TRUEPERELLA BERNARDIAE STRAIN ISOLATED FROM A DOG
| Autor: | Wyanda Arnafia, Siti Gusti Ningrum, Osama Sammra, Mazen Alssahen, Jörn-Peter Wickhorst, Christoph Lämmler, Ellen Prenger-Berninghoff, Markus Timke, Amir Abdulmawjood |
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| Jazyk: | Bosnian<br />English<br />Croatian<br />Serbian |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Veterinaria, Vol 66, Iss 2, Pp 66-71 (2017) |
| Druh dokumentu: | article |
| ISSN: | 0372-6827 2233-1360 |
| Popis: | The present study was designed to characterize phenotypically and genotypically the Trueperella bernardiae strain (T. bernardiae P5459/15) isolated from the purulent thelitis of a dog. The species identity could be confirmed by phenotypical investigations, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, and sequencing various molecular targets. Sequencing the 16S rDNA, the β subunit of bacterial RNA polymerase encoding gene rpoBand, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap revealed sequence similarities to the type strain T. bernardiae DSM 9152 of 99.6%, 99.3% and 99.1%, respectively. The present study is the first phenotypic and genotypic characterization of the T. bernardiae strain isolated from a dog. |
| Databáze: | Directory of Open Access Journals |
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