A method for the rapid isolation of virus from cultured cells
| Autor: | Byung-Whi Kong, Linda K. Foster, Douglas N. Foster |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 44, Iss 1, Pp 97-99 (2008) |
| Druh dokumentu: | article |
| ISSN: | 00011258 1940-9818 0736-6205 |
| DOI: | 10.2144/000112589 |
| Popis: | A simple and efficient collection method using hypotonic burst to isolate virions from infected cultured cells is described. Distilled water treatment of avian metapneumovirus (AMPV)-infected cells with thorough mixing and repeated pipeting was considerably faster for virion collection in avian cells compared to the widely used freeze-thaw (F-T) method (30 min vs. 3–4 h). This method was also more effective for virion collection. The total number of virions recovered from AMPV-infected immortal turkey turbinate cells by the novel water lysis method was 3-fold higher than by the F-T method. This simple water lysis method can be applied to virion collection for other RNA viruses such as the paramyxoviruses that are used to infect cultured cells. |
| Databáze: | Directory of Open Access Journals |
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