Kiwifruit (Actinidia chinensis) R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana

Autor: Ya-bin ZHANG, Wei TANG, Li-huan WANG, Ya-wen HU, Xian-wen LIU, Yong-sheng LIU
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Integrative Agriculture, Vol 18, Iss 2, Pp 417-427 (2019)
Druh dokumentu: article
ISSN: 2095-3119
DOI: 10.1016/S2095-3119(18)62127-6
Popis: Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses, such as drought, heat, cold, water logging and phytopathogens. Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement. Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog (AcMYB3R) whose expression was induced by drought, salinity and cold stress. In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE. The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress. The expressions of stress-responsive genes such as RD29A, RD29B, COR15A and RD22 were prominently up-regulated by ectopic expression of AcMYB3R. Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production.
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