| Autor: |
Li-Jing Zhu, Pan-Fei Hou, Ling Wang, Guang-Bo Zhang, Yan Xie, Xu-Dong Pan, Ting-Ting Chang |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
International Journal of Gerontology, Vol 6, Iss 3, Pp 187-191 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1873-9598 |
| DOI: |
10.1016/j.ijge.2012.01.013 |
| Popis: |
Background: CD4+CD25+Foxp3+ regulatory T (Treg) cells mediate immunosuppression and play an important role in tumor immune evasion. Studies have demonstrated that this cell population represents an aging-related change. It is not clear whether this change leads to higher tumor incidence in the elderly. We investigated changes in CD4+CD25+Foxp3+ Treg cells in relation to aging and tumor incidence. Methods: We set up a Lewis lung cancer model with 26 C57BL/6 female mice. The animals were divided into six groups: young healthy, middle-aged healthy, elderly healthy, young tumor, middle-aged tumor and elderly tumor. We evaluated changes in CD4+CD25+Foxp3+Treg cells in the spleen of all animals using a flow cytometry method. Levels of Foxp3 m RNA in splenocytes were measured using a real-time RT-PCR method. Results: The CD4+CD25+Foxp3+/CD4+ T cell ratio (t=2.23, p=0.032) and Foxp3 mRNA levels (t=3.26, p=0.0042) were higher in the tumor groups than in the healthy groups. In the healthy groups, there was a significant increase in CD4+CD25+Foxp3+ Treg cells on aging (F=47.70, p=0.000); elderly mice had a significantly greater population of CD4+CD25+Foxp3+ Treg cells in spleen compared to the younger groups. The highest population was observed in the elderly tumor group. The same trend was evident for Foxp3 mRNA (F=6.56, p=0.0090). Conclusions: The results suggest a close relationship between changes in CD4+CD25+Foxp3+ Treg cells and aging and lung tumor genesis and development. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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