CONTROLLED RATE FREEZING OF CANINE SPERMATOZOA1 USING A PROGRAMMABLE FREEZER
| Autor: | S.R. Mohanachandran Nair, J. Kalatharan, S.A. Asokan, P. Sridevi, J. Rajasekaran |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | Journal of Veterinary and Animal Sciences, Vol 29, Iss 1, Pp 36-38 (1998) |
| Druh dokumentu: | article |
| ISSN: | 0971-0701 2582-0605 |
| Popis: | A well defined slow freezing rate using a controlled rate freezer was used for cryopreservation of canine spermatozoa using Tris-egg yolk extender. Viability and membrane integrity of spermatozoa were assessed following freez-thawing. The freezing rate of -2 ° C min-' from 5 ° C to -15 ° C and -5 ° C min-' from -15 ° C to -40 ° C and -10 ° C min-' from -40 ° C to -70 ° C resulted in acceptable level of fertile spermatozoa following freez-thawing. |
| Databáze: | Directory of Open Access Journals |
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