Mesenchymal Stromal Cells Isolated from Irradiated Human Skin Have Diminished Capacity for Proliferation, Differentiation, Colony Formation, and Paracrine Stimulation

Autor: Maxwell B. Johnson, Solmaz Niknam‐Bienia, Vinaya Soundararajan, Brandon Pang, Eunson Jung, Daniel J. Gardner, Xingtian Xu, Sun Y. Park, Charles Wang, Xin Chen, Regina Y. Baker, Mei Chen, Young‐Kwon Hong, Wei Li, Alex K. Wong
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Stem Cells Translational Medicine, Vol 8, Iss 9, Pp 925-934 (2019)
Druh dokumentu: article
ISSN: 2157-6580
2157-6564
DOI: 10.1002/sctm.18-0112
Popis: Abstract Ionizing radiation, commonly used in the treatment of solid tumors, has unintended but deleterious effects on overlying skin and is associated with chronic nonhealing wounds. Skin‐derived mesenchymal stromal cells (SMSCs) are a pluripotent population of cells that are critically involved in skin homeostasis and wound healing. The aim of this study was to isolate and functionally characterize SMSCs from human skin that was previously irradiated as part of neoadjuvant or adjuvant cancer therapy. To this end, SMSCs were isolated from paired irradiated and nonirradiated human skin samples. Irradiated SMSCs expressed characteristic SMSC markers at lower levels, had disorganized cytoskeletal structure, and had disordered morphology. Functionally, these cells had diminished proliferative capacity and substantial defects in colony‐forming capacity and differentiation in vitro. These changes were associated with significant differential expression of genes known to be involved in skin physiology and wound healing. Conditioned media obtained from irradiated SMSCs affected fibroblast but not endothelial cell proliferation and migration. These results suggest that in situ damage to SMSCs during neoadjuvant or adjuvant radiation may play a critical role in the pathogenesis of slow or nonhealing radiation wounds. Stem Cells Translational Medicine 2019;8:925&934
Databáze: Directory of Open Access Journals
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