Open-source and low-cost miniature microscope for on-site fluorescence detection

Autor: Michio Kawai, Haruka Oda, Hisatoshi Mimura, Toshihisa Osaki, Shoji Takeuchi
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: HardwareX, Vol 19, Iss , Pp e00545- (2024)
Druh dokumentu: article
ISSN: 2468-0672
DOI: 10.1016/j.ohx.2024.e00545
Popis: The development of a compact and affordable fluorescence microscope can be a formidable challenge for growing needs in on-site testing and detection of fluorescent labeled biological systems, especially for those who specialize in biology rather than in engineering. In response to such a situation, we present an open-source miniature fluorescence microscope using Raspberry Pi. Our fluorescence microscope, with dimensions of 19.2 × 13.6 × 8.2 cm3 (including the display, computer, light-blocking case, and other operational requirements), not only offers cost-effectiveness (costing less than $500) but is also highly customizable to meet specific application needs. The 12.3-megapixel Raspberry Pi HQ Camera captures high-resolution imagery, while the equipped wide-angle lens provides a field of view measuring 21 × 15 mm2. The integrated wireless LAN in the Raspberry Pi, along with software-controllable high-powered fluorescence LEDs, holds potential for a wide range of applications. This open-source fluorescence microscope offers biohybrid sensor developers a versatile tool to streamline unfamiliar mechanical design tasks and open new opportunities for on-site fluorescence detections.
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