Investigation of Blastocystis hominis Frequency in Patients with Diabetes by Microscopy and Conventional PCR Methods
Autor: | Maksut Şahin, Zeynep Taş Cengiz, Ahmed Galip Halidi, Selahattin Aydemir |
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Jazyk: | angličtina |
Rok vydání: | 2023 |
Předmět: | |
Zdroj: | Iranian Journal of Parasitology, Vol 18, Iss 2 (2023) |
Druh dokumentu: | article |
ISSN: | 1735-7020 2008-238X |
DOI: | 10.18502/ijpa.v18i2.13186 |
Popis: | Background: We aimed to determine the frequency and subtype of B. hominis in diabetic patients. Methods: One hundred and fifty diabetic patients and 100 healthy people without any chronic disease were included in the study. Stool samples were analyzed by native-Lugol, condensation, trichrome staining and PCR methods. Results: İn 150 patients with diabetes; B. hominis was detected in 38 (25.3%) by PCR, in 34 (22.7%) by native-Lugol and trichrome staining. In the control group, 14 (14%) out of 100 subjects were positive by PCR, and 10 (10%) were positive by native-Lugol and trichrome staining. In the statistical evaluation, a significant difference was found between gender (P=0.023), age (P=0.045; ≤35 and >35 comparison), duration of diabetes (P=0.04), the HbA1c value (P=0.023; |
Databáze: | Directory of Open Access Journals |
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