Investigation of Blastocystis hominis Frequency in Patients with Diabetes by Microscopy and Conventional PCR Methods

Autor: Maksut Şahin, Zeynep Taş Cengiz, Ahmed Galip Halidi, Selahattin Aydemir
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Iranian Journal of Parasitology, Vol 18, Iss 2 (2023)
Druh dokumentu: article
ISSN: 1735-7020
2008-238X
DOI: 10.18502/ijpa.v18i2.13186
Popis: Background: We aimed to determine the frequency and subtype of B. hominis in diabetic patients. Methods: One hundred and fifty diabetic patients and 100 healthy people without any chronic disease were included in the study. Stool samples were analyzed by native-Lugol, condensation, trichrome staining and PCR methods. Results: İn 150 patients with diabetes; B. hominis was detected in 38 (25.3%) by PCR, in 34 (22.7%) by native-Lugol and trichrome staining. In the control group, 14 (14%) out of 100 subjects were positive by PCR, and 10 (10%) were positive by native-Lugol and trichrome staining. In the statistical evaluation, a significant difference was found between gender (P=0.023), age (P=0.045; ≤35 and >35 comparison), duration of diabetes (P=0.04), the HbA1c value (P=0.023;
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