High production of pro-inflammatory cytokines by maternal blood mononuclear cells is associated with reduced maternal malaria but increased cord blood infection

Autor: Carlota Dobaño, Tamara Berthoud, Maria Nelia Manaca, Augusto Nhabomba, Caterina Guinovart, Ruth Aguilar, Arnoldo Barbosa, Penny Groves, Mauricio H. Rodríguez, Alfons Jimenez, Lazaro M. Quimice, John J. Aponte, Jaume Ordi, Denise L. Doolan, Alfredo Mayor, Pedro L. Alonso
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Malaria Journal, Vol 17, Iss 1, Pp 1-13 (2018)
Druh dokumentu: article
ISSN: 1475-2875
DOI: 10.1186/s12936-018-2317-2
Popis: Abstract Background Increased susceptibility to malaria during pregnancy is not completely understood. Cellular immune responses mediate both pathology and immunity but the effector responses involved in these processes have not been fully characterized. Maternal and fetal cytokine and chemokine responses to malaria at delivery, and their association with pregnancy and childhood outcomes, were investigated in 174 samples from a mother and child cohort from Mozambique. Peripheral and cord mononuclear cells were stimulated with Plasmodium falciparum lysate and secretion of IL-12p70, IFN-γ, IL-2, IL-10, IL-8, IL-6, IL-4, IL-5, IL-1β, TNF, TNF-β was quantified in culture supernatants by multiplex flow cytometry while cellular mRNA expression of IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10 and IL-13 was measured by quantitative PCR. Results Higher concentrations of IL-6 and IL-1β were associated with a reduced risk of P. falciparum infection in pregnant women (p 0.5, p
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