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ObjectiveTo explore the role of hippocampal microglia activation and related proinflammatory factors in depression-like behavior induced by chronic stress in rats with ischemic stroke.MethodsA total of seventy-five healthy male Sprague-Dawley rats were used to establish a right middle cerebral artery occlusion (MCAO) model with 70 min ischemia. One week after MCAO, rats were assigned to stroke and "stroke + stress" groups according to the SPSS random number generator. And according to time course, the former was divided into 1-week stroke, 2-week stroke and 4-week stroke groups, while the latter was divided into "2-week stroke + 1-week stress" (referred to as "1-week stress") and "4-week stroke + 3-week stress" (as "3-week stress") groups, with 15 rats in each group. "Chronic unpredictable mild stress plus isolation" was used in the stress group. The body weight, open field test (OFT), novelty-suppressed feeding test (NSFT) and sugar preference index (SPI) of rats were evaluated at each time point. Residual brain volume ratio was detected by TTC staining. The expression of Iba-1, IL-1β and IL-18 in the affected hippocampus was detected by Western blot. The fluorescencs intensity of Iba-1 in the CA1 region of the affected hippocampus was detected by immunofluorescence staining.ResultsTwo weeks after MCAO, compared with the 2-week stroke group, the body weight and counts of rearing and grid crossing of OFT decreased in the 1-week stress group (all PPP>0.05). Western blot showed that, compared with the 4-week stroke group or 1-week stress group, the expression of Iba-1, IL-1β and IL-18 in the affected hippocampus was significantly higher in the 3-week stress group (all PPPP |
