Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat
| Autor: | Gabré Kemp, Carl W. Bergmann, Ron Clay, Amie J. Van der Westhuizen, Zacharias A. Pretorius |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Molecular Plant-Microbe Interactions, Vol 16, Iss 11, Pp 955-961 (2003) |
| Druh dokumentu: | article |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/MPMI.2003.16.11.955 |
| Popis: | Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP. |
| Databáze: | Directory of Open Access Journals |
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