CCMetagen: comprehensive and accurate identification of eukaryotes and prokaryotes in metagenomic data

Autor: Vanessa R. Marcelino, Philip T. L. C. Clausen, Jan P. Buchmann, Michelle Wille, Jonathan R. Iredell, Wieland Meyer, Ole Lund, Tania C. Sorrell, Edward C. Holmes
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Genome Biology, Vol 21, Iss 1, Pp 1-15 (2020)
Druh dokumentu: article
ISSN: 1474-760X
DOI: 10.1186/s13059-020-02014-2
Popis: Abstract There is an increasing demand for accurate and fast metagenome classifiers that can not only identify bacteria, but all members of a microbial community. We used a recently developed concept in read mapping to develop a highly accurate metagenomic classification pipeline named CCMetagen. The pipeline substantially outperforms other commonly used software in identifying bacteria and fungi and can efficiently use the entire NCBI nucleotide collection as a reference to detect species with incomplete genome data from all biological kingdoms. CCMetagen is user-friendly, and the results can be easily integrated into microbial community analysis software for streamlined and automated microbiome studies.
Databáze: Directory of Open Access Journals