Metformin increases the expression of proinflammatory cytokines and inhibits supraspinatus fatty infiltration

Autor: Jong Pil Yoon, Sung-Jin Park, Dong-Hyun Kim, Seok Won Chung
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Orthopaedic Surgery and Research, Vol 18, Iss 1, Pp 1-8 (2023)
Druh dokumentu: article
ISSN: 1749-799X
DOI: 10.1186/s13018-023-04163-z
Popis: Abstract Background After a rotator cuff (RC) tendon tear, the supraspinatus (SS) inflammatory response induces fatty infiltration (FI). Metformin has the effect of regulating the initial inflammatory response of atrophic muscles. Therefore, this study aimed to investigate the effect of metformin use on modulating the expression of proinflammatory cytokines and SS FI in an acute RC tear rat model. Methods This study used 26 male Sprague–Dawley rats. Animals were randomly divided into two groups: The metformin group received metformin for 5 days after cutting the RC tendon, and the control group was administered only with saline after cutting the tendon. Metformin 50 mg/kg was intraperitoneally injected for 5 days. Three rats in each group were sacrificed 5 days after SS tendon rupture surgery, and 10 rats in each group were sacrificed 14 days after surgery. The SS was sampled 5 days after SS tendon tear surgery, and the expression of proinflammatory cytokines was measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). On day 14 after sampling, histological analysis of the SS was performed using hematoxylin and eosin, Masson’s trichrome, and picrosirius red staining. Results On day 5 of surgery, the expression values of interferon gamma (increased 7.2-fold, P
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