| Autor: |
Wirdatun Nafisah, Nadia Wahyuningsih, Muhammad Faiz Maulana, Shella Zahra Kumala Azmi, Muhaimin Rifa’i, Nashi Widodo, Muhammad Sasmito Djati |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of Pharmacy & Pharmacognosy Research, Vol 11, Iss 4, Pp 674-682 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
0719-4250 |
| DOI: |
10.56499/jppres23.1604_11.4.674 |
| Popis: |
Context: Triple-negative breast cancer (TNBC) is a type of breast cancer with the highest aggressiveness and malignancy characteristics. Aims: To evaluate the possible anticancer potential of Cyperus rotundus rhizome extract (CRE) against 4T1-tumor-bearing mice. Methods: C. rotundus rhizome was extracted using maceration methods with ethanol. C. rotundus extract (CRE) was then determined the total phenolic (TPC) and flavonoid content (TFC) using colorimetric analysis. The cytotoxic activity of CRE against 4T1 cells was carried out operating WST-1 assay, and the IC50 value was then used for in vivo study. The 4T1-tumor-bearing mice were treated with CRE at 72.5, 145, and 290 mg/kg body weight (BW) for two weeks or treated with cisplatin once a week for two weeks (4 mg/kg BW). The analysis of IL-2 cytokines production and the activation of CD4 and CD8 T cells was assessed using flow cytometry analysis. Histopathological analysis with hematoxylin and eosin (HE) staining was applied to determine the outcome of CRE on breast cancer tissue. Results: CRE indicated a higher TPC (76.97 mg GAE/g) value than TFC (29.37 mg QE/g). This study demonstrated a significant reduction of IL-2 cytokines and CD4 T cell activation in treated groups than in the cancer group (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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