Construction and Approval of the Test-System for the Detection of Antibodies to Anthrax Agent Using Indirect Fluorescent Immunoassay
| Autor: | L. Yu. Aksenova, A. G. Ryazanova, E. V. Zhdanova, O. I. Tsygankova, N. P. Buravtseva, E. I. Eremenko, I. S. Tyumentseva, O. I. Kogotkova, A. A. Zuenko, A. N. Kulichenko |
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| Jazyk: | ruština |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Проблемы особо опасных инфекций, Vol 0, Iss 4, Pp 76-78 (2013) |
| Druh dokumentu: | article |
| ISSN: | 0370-1069 2658-719X |
| DOI: | 10.21055/0370-1069-2013-4-76-78 |
| Popis: | Constructed and validated has been the test-system for the detection of antibodies to anthrax agent using indirect fluorescent immunoassay. The panel consists of antigen preparation, positive control sample, negative control, and FITS-labeled rabbit antibodies. Studied have been 10 acapsular B. anthracis strains with different plasmid content and colony morphology. B. anthracis vaccine strain STI-1 is demonstrated to be the optimal one as antigen preparation. Sensitivity of this test-system is not less than 1:40, the working dilution of FITC-conjugate being 1:16. Specificity of the panel has been studied using 100 sera of healthy donors. Approved is 100 % reproducibility of the technique at different time intervals, as well as when carried by different specialists. The test-system allows for confirmation of anthrax diagnosis in humans. |
| Databáze: | Directory of Open Access Journals |
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