Autor: |
Aleksandra Ponitka, Aurelia Ślusarkiewicz-Jarzina |
Jazyk: |
angličtina |
Rok vydání: |
2011 |
Předmět: |
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Zdroj: |
Acta Societatis Botanicorum Poloniae, Vol 78, Iss 3, Pp 209-213 (2011) |
Druh dokumentu: |
article |
ISSN: |
2083-9480 |
DOI: |
10.5586/asbp.2009.026 |
Popis: |
The effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regeneration on medium 190-2 was tested, in relation to different ELS culture conditions. The highest rate of green plants per 100 ELS (3.3 - 42.4) was produced by incubation at 22oC in the dark for the first two weeks. Among 36 green regenerants, 28 (77.8%) were haploid and 8 (22.2%) were spontaneous doubled haploids, fully fertile. After colchicine treatment of haploid plants, 19 were partially fertile and set from 1 to 15 seed per panicle. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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