Enrichment of CpG island shore region hypermethylation in epigenetic breast field cancerization

Autor: Meghan E. Muse, Alexander J. Titus, Lucas A. Salas, Owen M. Wilkins, Chelsey Mullen, Kelly J. Gregory, Sallie S. Schneider, Giovanna M. Crisi, Rahul M. Jawale, Christopher N. Otis, Brock C. Christensen, Kathleen F. Arcaro
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Epigenetics, Vol 15, Iss 10, Pp 1093-1106 (2020)
Druh dokumentu: article
ISSN: 1559-2294
1559-2308
15592294
DOI: 10.1080/15592294.2020.1747748
Popis: While changes in DNA methylation are known to occur early in breast carcinogenesis and the landscape of breast tumour DNA methylation is profoundly altered compared with normal tissue, there have been limited efforts to identify DNA methylation field cancerization effects in histologically normal breast tissue adjacent to tumour. Matched tumour, histologically normal tissue of the ipsilateral breast (ipsilateral-normal), and histologically normal tissue of the contralateral breast (contralateral-normal) were obtained from nine women undergoing bilateral mastectomy. Laser capture microdissection was used to select epithelial cells from normal tissue, and neoplastic cells from tumour for genome-scale measures of DNA methylation with the Illumina HumanMethylationEPIC array. We identified substantially more CpG loci that were differentially methylated between contralateral-normal and tumour (63,271 CpG loci q
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