| Autor: |
Gillant Flavie, Moreau Julien, Richly Maximilian U., Alexandrou Antigoni, Perronet Karen, Westbrook Nathalie |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of the European Optical Society-Rapid Publications, Vol 19, Iss 1, p 28 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1990-2573 |
| DOI: |
10.1051/jeos/2023026 |
| Popis: |
Mechanical forces play an important role in the behaviour of cells, from differentiation to migration and the development of diseases. Optical tweezers provide a quantitative tool to study these forces and must be combined with other tools, such as phase contrast and fluorescence microscopy. Detecting the retro-reflected trap beam is a convenient way to monitor the force applied by optical tweezers, while freeing top access to the sample. Accurate in situ calibration is required especially for single cells close to a surface where viscosity varies rapidly with height. Here, we take advantage of the well contrasted interference rings in the back focal plane of the objective to find the height of a trapped bead above a cover slip. We thus map the viscous drag dependence close to the surface and find agreement between four different measurement techniques for the trap stiffness down to 2 μm above the surface. Combining this detection scheme with phase contrast microscopy, we show that the phase ring in the back focal plane of the objective must be deported in a conjugate plane on the imaging path. This simplifies implementation of optical tweezers in combination with other techniques for biomechanical studies. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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