In vitro regeneration of Vitellaria paradoxa from shoot tip explants

Autor: Nusrat Tsemah Afful, Iddrisu Abdulai, Elaine Azu, Wilfred Elegba, Clement Annor, Christian Akama, Kwame Asare, John Dentey, Harry M. Amoatey
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: BioTechnologia, Vol 103, Iss 1, Pp 71-79 (2022)
Druh dokumentu: article
ISSN: 0860-7796
2353-9461
DOI: 10.5114/bta.2022.113917
Popis: Shea tree (Vitellaria paradoxa) is an essential tree crop with great potential economic value mainly because of its seed oil (shea butter) which is of high demand for manufacturing assorted products in food, cosmetic, and rubber industries. Propagation of this species is, however, hindered by relative unavailability of seed (nuts), erratic seed germination, a long vegetative phase, and latex exudation from cuttings. Thus, another method of propagation through in vitro culture is recommended for rapid multiplication of shea genotypes for large-scale cultivation. In the present study, the effects of two cytokinins, namely, 6-benzylaminopurine (BAP) and kinetin (KIN), and one auxin, namely 1-naphthaleneacetic acid (NAA), on shoot and/or root induction in vitro were asses sed at various combinations/concentrations. The inclusion of these growth regulators in the culture medium significantly improved (P
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