| Autor: |
Akiko Obata, Kazuma Mori, Keiichi Inukai, Katsuya Kato, Gowsihan Poologasundarampillai, Toshihiro Kasuga |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Frontiers in Materials, Vol 7 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2296-8016 |
| DOI: |
10.3389/fmats.2020.00033 |
| Popis: |
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), P(3HB-4HB), and siloxane-doped vaterite (SiV) composite fibrous scaffolds with 3D cotton-wool-like structure were developed using an electrospinning system for use in bone tissue regeneration. Scaffolds exhibited a significantly larger fiber-fiber separation distribution than non-woven fiber mats as observed with micro-computed tomographic studies. Coating the hydrophobic P(3HB-4HB)/SiV fibers with imogolite nanotubes (INT), aluminum silicate nanotubes, made the 3D construct hydrophilic and improved water penetration into the 3D structure (~2 s). Coating efficacy was confirmed by the detection of aluminum on the surface of fibers using scanning electron microscopy (SEM) energy dispersive spectroscopy (EDS). Dissolution experiments showed increased release of silicate ions in cell culture medium which can improve migration and mineralization of osteogenic cells inside of the 3D structure. The coating also contributed to an enhanced adhesion and migration of osteoblast-like cells (SaOS-2) within the 3D construct. The differentiation and mineralization of the cells were not affected by the coating. The coating for such cotton-wool-like structured scaffolds was effective for an enhancement of cell functions on early stages of culture. Thus, the developed materials with 3D structure, flexibility, silicate-ion release ability, and cell compatibility are expected to be good candidate materials for bone tissue regeneration. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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