| Autor: |
Antti I. Nykänen, Andrea Mariscal, Allen Duong, Catalina Estrada, Aadil Ali, Olivia Hough, Andrew Sage, Bonnie T. Chao, Manyin Chen, Hemant Gokhale, Hongchao Shan, Xiaohui Bai, Guan Zehong, Jonathan Yeung, Tom Waddell, Tereza Martinu, Stephen Juvet, Marcelo Cypel, Mingyao Liu, John E. Davies, Shaf Keshavjee |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss , Pp 184-197 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2329-0501 |
| DOI: |
10.1016/j.omtm.2021.05.018 |
| Popis: |
Ex vivo lung perfusion (EVLP) is an excellent platform to apply novel therapeutics, such as gene and cell therapies, before lung transplantation. We investigated the concept of human donor lung engineering during EVLP by combining gene and cell therapies. Premodified cryopreserved mesenchymal stromal cells with augmented anti-inflammatory interleukin-10 production (MSCIL-10) were administered during EVLP to human lungs that had various degrees of underlying lung injury. Cryopreserved MSCIL-10 had excellent viability, and they immediately and efficiently elevated perfusate and lung tissue IL-10 levels during EVLP. However, MSCIL-10 function was compromised by the poor metabolic conditions present in the most damaged lungs. Similarly, exposing cultured MSCIL-10 to poor metabolic, and especially acidic, conditions decreased their IL-10 production. In conclusion, we found that “off-the-shelf” MSCIL-10 therapy of human lungs during EVLP is safe and feasible, and results in rapid IL-10 elevation, and that the acidic target-tissue microenvironment may compromise the efficacy of cell-based therapies. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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