BNIP3 Upregulation Characterizes Cancer Cell Subpopulation With Increased Fitness and Proliferation

Autor: Yanyan Zhu, Bowang Chen, Junya Yan, Wendi Zhao, Pengli Dou, Na Sun, Yaokai Wang, Xiaoyun Huang
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in Oncology, Vol 12 (2022)
Druh dokumentu: article
ISSN: 2234-943X
DOI: 10.3389/fonc.2022.923890
Popis: BNIP3 is a BH3-only protein with both pro-apoptotic and pro-survival roles depending on the cellular context. It remains unclear how BNIP3 RNA level dictates cell fate decisions of cancer cells. Here, we undertook a quantitative analysis of BNIP3 expression and functions in single-cell datasets of various epithelial malignancies. Our results demonstrated that BNIP3 upregulation characterizes cancer cell subpopulations with increased fitness and proliferation. We further validated the upregulation of BNIP3 in liver cancer 3D organoid cultures compared with 2D culture. Taken together, the combination of in silico perturbations using public single-cell datasets and experimental cancer modeling using organoids ushered in a new approach to address cancer heterogeneity.
Databáze: Directory of Open Access Journals