A new immunochemistry process that transform a non-immunogenic crotamine-like antigen from rattlesnake (Crotalus durissus cumanensis) venom, in immunogenic to produce anti-crotamine-like antibodies.
| Autor: | María Magdalena Pulido-Mendez, María Eugenia Acosta, Alexis Rodríguez-Acosta |
|---|---|
| Jazyk: | English<br />Spanish; Castilian |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Revista Científica, Vol 30, Iss 4 (2021) |
| Druh dokumentu: | article |
| ISSN: | 0798-2259 2521-9715 |
| Popis: | The making of antibodies in animals can be demanding due to that several antigens, mostly of low molecular masses, provoke imperceptible immune response or are even totally non-immunogenic. The transformation of non-immunogenic molecules into effective antigens represent an important immunological tasks. The crotamine from the rattlesnake Crotalus durissus cumanensis snake venom was purified by a Mono S HR 10/10 chromatography column and used to immunise C57/B mice, after to be polymerised with glutaraldehyde. The murine polyclonal antibodies directed against native crotamine-like (NCL) treated with glutaraldehyde and their product crotamine-like polymer (CLP) were generated by immunisation injecting CLP via lymph node cells. These antibodies were capable of detecting CLP in an enzyme-linked immunosorbent assay. The SDS-PAGE of NCL and CLP showed bands of molecular masses ~ 3 kDa and ~18 kDa, respectively. These results offer evidence that the polyclonal antibodies recognise specific putative original and post-polymerisation epitopes on the CLP molecule, which were maintained following the process of polymerisation. The results are discussed in relation to the preservation of a functional post-polymerisation epitopes on CLP. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|