| Autor: |
Ting-ting Liu, Han Hou, Jiu-lin Du |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 2, Iss 1, Pp 100388- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100388 |
| Popis: |
Summary: Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ activity and morphology of growing brain ETCs in larval zebrafish.For complete details on the use and execution of this protocol, please refer to Liu et al. (2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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