LC–MS/MS-based quantification of tryptophan, kynurenine, and kynurenic acid in human placental, fetal membranes, and umbilical cord samples

Autor: Bruno Pedraz-Petrozzi, Marta Marszalek-Grabska, Anna Kozub, Klaudia Szalaj, Alicja Trzpil, Anna Stachniuk, Eva Kathrin Lamadé, Maria Gilles, Michael Deuschle, Waldemar A. Turski, Emilia Fornal
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Druh dokumentu: article
ISSN: 2045-2322
21834539
DOI: 10.1038/s41598-023-39774-3
Popis: Abstract Tryptophan breakdown metabolites formed along the kynurenine pathway play a significant role in pregnancy and fetal development. To understand their involvement, it is crucial to quantify the levels of tryptophan (TRP), kynurenine (KYN), and kynurenic acid (KYNA) in relevant biological samples such as the placenta, fetal membranes, and umbilical cord. This study used liquid chromatography-tandem mass spectrometry (LC–MS/MS) to determine TRP, KYN, and KYNA levels. The LC–MS/MS method was optimized for high sensitivity and specificity, demonstrating good reproducibility with a precision of
Databáze: Directory of Open Access Journals
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