Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
| Autor: | YUDHIE ISTIANTO, RADEN SETYO ADJI KOESOEMOWIDODO, YOSHIO WATANABE, HARDANING PRANAMUDA, BAMBANG MARWOTO |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Microbiology Indonesia, Vol 6, Iss 1 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1978-3477 2087-8575 |
| DOI: | 10.5454/mi.6.1.7 |
| Popis: | Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively. |
| Databáze: | Directory of Open Access Journals |
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