| Autor: |
Diana Munguia-Ramos, Luis Fernando Xancal-Salvador, Verónica Esteban-Kenel, Narciso Ortiz-Conchi, Ricardo Antonio Jaimes-Aquino, Miguel Mendoza-Rojas, Axel Cervantes-Sánchez, Steven Méndez-Ramos, Hector Orlando Rivera-Villegas, Sandra Rajme-Lopez, Karla Maria Tamez-Torres, Carla Marina Roman-Montes, Areli Martínez-Gamboa, Miriam Bobadilla del-Valle, Jose Sifuentes-Osornio, Alfredo Ponce-de-Leon, Maria Fernanda Gonzalez-Lara, Bernardo Alfonso Martinez-Guerra |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Antibiotics, Vol 13, Iss 11, p 1105 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2079-6382 |
| DOI: |
10.3390/antibiotics13111105 |
| Popis: |
Background: Infections due to carbapenem-resistant Gram-negative bacteria are emerging as an important challenge in health-care settings and a growing concern worldwide. Lateral flow immunoassay NG-Test® CARBA 5 can detect the five most reported carbapenemases (KPC, OXA-48-like, VIM, IMP, and NDM). Direct testing of positive blood cultures could reduce time to detection. This study aims to validate and report on the diagnostic yield of a novel method for carbapenemase detection in positive blood culture vials using NG-Test® CARBA 5. Methods: We implemented an investigator-developed method for the direct testing of positive blood cultures using NG-Test® CARBA 5. We compared results between genotypic, phenotypic, and direct NG-Test® CARBA 5 in blood. Results: A total of 32 isolates were tested (21 Enterobacterales and 11 Pseudomonas aeruginosa). Genotypic testing detected 23 carbapenemases. When comparing the results of NG-Test® CARBA 5 in blood with genotypic testing, agreement was observed in 31/32 (97%) tests. The sensitivity, specificity, positive predictive value, and negative predictive value of the NG-Test® CARBA 5 in blood were 93%, 100%, 100%, and 94%, respectively. Conclusions: Our method using NG-Test® CARBA 5 directly in blood culture samples presented an excellent diagnostic yield when compared to genotypic profiling and permits an accurate detection of carbapenemases. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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