| Autor: |
Mariko Yamamoto Kawai, Takeshi Yoshida, Tomoki Kato, Takuma Watanabe, Marina Kashiwagi, Shigeki Yamanaka, Hiromitsu Yamamoto, Shigeki Nagahiro, Tsutomu Iwamoto, Khan Masud, Kazuhiro Aoki, Kiyoshi Ohura, Kazumasa Nakao |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Materials, Vol 17, Iss 4, p 880 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1996-1944 |
| DOI: |
10.3390/ma17040880 |
| Popis: |
Background: Bone morphogenetic protein-2 (bmp-2) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the bmp-2 gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral bmp-2 gene expression plasmid vector (pCAGGS-bmp-2) and needle-type electrodes. Methods: We injected the bmp-2 gene with pCAGGS-bmp-2 into the skeletal muscles of rats’ legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the bmp-2 gene to skeletal muscles, making them potential biomaterials for repairing bone defects. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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