| Autor: |
S. Sh. Khayat, E. E. Bragina, E. A. Arifulin, E. M. Lazareva, T. M. Sorokina, L. F. Kurilo, V. B. Chernykh |
| Jazyk: |
ruština |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Андрология и генитальная хирургия, Vol 20, Iss 4, Pp 39-44 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2070-9781 |
| DOI: |
10.17650/2070-9781-2019-20-4-39-44 |
| Popis: |
The study objective is to analyze the content of spermatozoa with single and double-stranded DNA breaks in different age groups.Materials and methods. The level of DNA fragmentation was studied in 300 ejaculate samples obtained from 266 sub- or infertile men. The group 1 included 150 samples obtained from 131 patients under the age of 45 (21–44 years), the group 2 included 150 samples obtained from 135 patients above the age of 45 (45–68 years). Mean ages were 34.8 ± 3.9 and 48.6 ± 3.1 years, respectively. The number of sperm with fragmented DNA was evaluated using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method on ejaculate smears. The number of spermatozoa with >15 % of fragmented DNA was considered elevated. Standard semen analysis was performed in 117 and 97 men from the groups 1 and 2, respectively.Results. The number of sperm with fragmented DNA varied in ejaculated samples from 1.5 to 64.5 %. Mean number of sperm with DNA breaks in the group 1 (12.0 ± 6.0 %) was significantly lower than in the group 2 (16.1 ± 8.3 %, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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