| Autor: |
Jinling Wang, Di Ye, Shuangpeng Li, Xuan Lu, Yue Chi, Chengcheng Tang, Xiaoqing Zhou, Min Chen, Yunwen Zheng, Jianyun Ge, Qingjian Zou |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Stem Cell Research, Vol 81, Iss , Pp 103543- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1873-5061 |
| DOI: |
10.1016/j.scr.2024.103543 |
| Popis: |
Current methodologies for hepatocyte induction from human induced pluripotent stem cells (hiPSCs) have limited efficacy due to lack of a functional hepatocyte reporter. To address this, we developed an endogenous albumin (ALB)-sfGFP reporter system in hiPSCs using homologous directed recombination (HDR)-mediated knock-in. The hiPSCs maintained the characteristic morphology, pluripotency, and normal karyotype while demonstrating successful differentiation into all three germ layers both in vitro and in vivo. Co-expression of EGFP and ALB was observed in the derived hepatocyte-like cells (HLCs). This reporter system holds promise for functional hepatocyte induction. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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